Dual TCR-a Expression on Mucosal-Associated Invariant T Cells as a Potential Confounder of TCR Interpretation

S Suliman; L Kjer-Nielsen; SK Iwany; KL Tamara; L Loh; L Grzelak; K Kedzierska; TA Ocampo; AJ Corbett; J McCluskey; J Rossjohn; SR León; R Calderon; L Lecca-Garcia; MB Murray; DB Moody; IV Rhijn

Journal article

Dual TCR-a Expression on Mucosal-Associated Invariant T Cells as a Potential Confounder of TCR Interpretation

S Suliman, L Kjer-Nielsen, SK Iwany, KL Tamara, L Loh, L Grzelak, K Kedzierska, TA Ocampo, AJ Corbett, J McCluskey, J Rossjohn, SR León, R Calderon, L Lecca-Garcia, MB Murray, DB Moody, IV Rhijn

Journal of Immunology | AMER ASSOC IMMUNOLOGISTS | Published : 2022

DOI: 10.4049/jimmunol.2100275

Abstract

Mucosal-associated invariant T (MAIT) cells are innate-like T cells that are highly abundant in human blood and tissues. Most MAIT cells have an invariant TCRa-chain that uses T cell receptor a-variable 1-2 (TRAV1-2) joined to TRAJ33/20/12 and recognizes metabolites from bacterial riboflavin synthesis bound to the Ag-presenting molecule MHC class I related (MR1). Our attempts to identify alternative MR1-presented Ags led to the discovery of rare MR1-restricted T cells with non-TRAV1-2 TCRs. Because altered Ag specificity likely alters affinity for the most potent known Ag, 5-(2-oxopropylideneamino)-6-D-ribitylaminouracil (5-OP-RU), we performed bulk TCRa- and TCRb-chain sequencing and single..

View full abstract

University of Melbourne Researchers

Liyen Loh Author

Ludivine Grzelak Author

Katherine Kedzierska Author

Alexandra Corbett Author

Related Projects (1)

Understanding recognition of vitamin B-based antigens by the immune system

This project aims to evaluate the range of molecules that can stimulate vitamin B-reactive T cells in mammals and amphibians, and the degree..

Grants

Awarded by National Institutes of Health

Funding Acknowledgements

This work was supported by National Institutes of Health TB Research Unit Network Grants U19 AI111224 and R01AI049313. J.R. and J.M. are supported by a grant fromthe National Institutes of Health, Grant R01 AI 148407-01 A1. S.S. received the Justice, Equity, Diversity and Inclusion award, which provided free language editorial service for the manuscript. A.J.C. is supported by a future fellowship (FT160100083) from the Australian Research Council, an investigator grant from the National Health and Medical Research Council (1193745) , and a Dame Kate Campbell Fellowship from the University of Melbourne. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. K.K. was supported by National Health and Medical Research Council Leadership Investigator Grant 1173871.